Here you can post pictures and videos to show others.
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JimT
- Posts: 3247
- Joined: Fri Oct 24, 2014 1:57 pm
#1
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by JimT » Tue Dec 15, 2015 8:53 pm
Here are two images of a cross section of a tomato stem cut with my home made microtome. Stained with methylene blue. Unfortunately, some air bubbles still visible.
Epidermis, cortex, xylem, and pith visible.
4X Obj.
10X Obj.
![Image](http://i571.photobucket.com/albums/ss152/jtallon2/IMG_0018-and-19-100X-JPEG_zps961nzg2z.jpg)
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mrsonchus
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#2
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by mrsonchus » Tue Dec 15, 2015 11:13 pm
Very nice, the Methylene blue has added useful contrast too. Living tissue always looks so colourful - I like it a lot.
![Smile :)](./images/smilies/icon_e_smile.gif)
John B
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JimT
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#3
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by JimT » Wed Dec 16, 2015 12:00 am
Thanks John B.
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rnabholz
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#4
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by rnabholz » Wed Dec 16, 2015 12:05 am
Hey Jim,
Great Shots- love the color and super detail, really fun to explore with the eye.
Of course the eye candy starts with your knife- really good sections.
Well done sir.
Rod
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KurtM
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#5
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by KurtM » Wed Dec 16, 2015 1:56 am
YEAH Rod, where's your microtome stuff?? You were posting some pretty darn decent images last I saw (chopping up the wife's houseplants, hahaha).
JimT, sorry for that outburst. Your sections are impressive and motivational. One of these days I will be inspired sufficiently to have a go with the gear I already got but never even tried yet. Thank you for posting, and I agree: well done sir!
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75RR
- Posts: 8207
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- Location: Estepona, Spain
#6
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by 75RR » Wed Dec 16, 2015 2:08 am
Nice. Good range of colours.
Last edited by
75RR on Wed Dec 16, 2015 10:39 am, edited 2 times in total.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
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zzffnn
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- Location: Houston, Texas, USA
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#7
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by zzffnn » Wed Dec 16, 2015 4:19 am
Great result and very nice image!!
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Crater Eddie
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- Location: Illinois USA
#8
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by Crater Eddie » Wed Dec 16, 2015 2:07 pm
That looks very good. Makes a person want to buy or build a microtome. Dang it.
CE
Olympus BH-2 / BHTU
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)
Cameras: Canon T3i, Olympus E-P1 MFT, Amscope 3mp USB
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mrsonchus
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- Location: Cumbria, UK
#9
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by mrsonchus » Wed Dec 16, 2015 2:18 pm
Crater Eddie wrote:That looks very good. Makes a person want to buy or build a microtome. Dang it.
CE
If you've not got one Eddie - go buy one - stat! You know it makes sense......it's Cristmas and you deseerrrrvvveeeee onee....
Everyone should own at least a hand microtome, it should be the Law!
![Very Happy :D](./images/smilies/icon_e_biggrin.gif)
John B
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gekko
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#10
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by gekko » Wed Dec 16, 2015 3:13 pm
JimT, great work, as usual: the sectioning, the staining, and the imaging.
mrsonchus wrote:If you've not got one Eddie - go buy one - stat! You know it makes sense......it's Cristmas and you deseerrrrvvveeeee onee....
I must disagree-- CE already got his BIG Christmas present for this year. Maybe next year, though
![Smile :)](./images/smilies/icon_e_smile.gif)
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JimT
- Posts: 3247
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#11
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by JimT » Wed Dec 16, 2015 3:38 pm
Thanks all.
CE already got his BIG Christmas present for this year. Maybe next year, though
I think CE has been a good boy and is worthy of two presents
![Smile :)](./images/smilies/icon_e_smile.gif)
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gekko
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#12
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by gekko » Wed Dec 16, 2015 5:47 pm
JimT wrote:Thanks all.
CE already got his BIG Christmas present for this year. Maybe next year, though
I think CE has been a good boy and is worthy of two presents
![Smile :)](./images/smilies/icon_e_smile.gif)
I agree wholeheartedly
![Smile :)](./images/smilies/icon_e_smile.gif)
.
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mrsonchus
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#13
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by mrsonchus » Wed Dec 16, 2015 9:02 pm
Too right! You can never have too many presents!
![Very Happy :D](./images/smilies/icon_e_biggrin.gif)
Plus, if Eddie gets one, we're all in for a treat with the pictures!
![Very Happy :D](./images/smilies/icon_e_biggrin.gif)
John B